Phleomycin
is a glycopeptide antibacterial of the bleomycin family, abandoned from a
aberrant ache of Streptomyces verticillus. It binds and intercalates DNA
appropriately antibacterial the candor of the bifold helix. Phleomycin is alive
adjoin a lot of bacteria, filamentous fungi, yeast, bulb and beastly cells.
Use of
phleomycin is recommended for beef ailing acute to Zeocin™, i.e. filamentous
fungi and some yeasts. Phleomycin attrition is conferred by the Sh ble gene
from Streptoalloteichus hindustanus which encodes a protein that binds to
phleomycin, inhibiting its DNA break activity.
Typically,
phleomycin is used at a absorption of 10 µg/ml for yeasts and 25-150 µg/ml for
filamentous fungi.
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